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ni nitriloacetic acid nta hispur agarose resin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ni nitriloacetic acid nta hispur agarose resin
    Ni Nitriloacetic Acid Nta Hispur Agarose Resin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hispur+ni-nta+resin/Agarose/bio_rxiv__64898__2026__04__09__717454-105-30-36
    Average 99 stars, based on 1 article reviews
    ni nitriloacetic acid nta hispur agarose resin - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title:
    Article Snippet: Cell debris was removed by centrifugation (32,000 g, 1 h, 4 °C), and the supernatant was directly loaded onto a HisPur Ni‐ NTA resin (Thermo Scientific) column containing 15 mL of packed resin pre‐equilibrated with the lysis buffer.

    Article Title: Supplementary Information for Structural insights into the regulation of protein-arginine kinase McsB by McsA
    Article Snippet: After complete cleavage of 6×His-Trx-McsB into 6×His-Trx and McsB, the protein solution was dialyzed in buffer A for 3 h and passed through HisPur Ni-NTA resin (Thermo Fisher Scientific, Waltham, MA, USA) to remove the 6×His-Trx tag.

    Article Title:
    Article Snippet: His purification was conducted using the HisPur Ni-NTA resin from Thermo Fisher and the His-Spin buffers from Zymo.

    Article Title: Programmable translational inhibition by a molecular glue-oligonucleotide conjugate
    Article Snippet: After cleavage, the mixture was incubated with HisPur Ni-NTA resin (Thermo Fisher Scientific; 1 mL slurry per liter of culture) for 10 min, and the flow-through containing tag-free eIF4A1 was collected.

    Article Title: Structure and functional analysis of CDNF-BiP interaction reveal a role in endoplasmic reticulum proteostasis regulation
    Article Snippet: The lysate was centrifuged at 20,000 × g for 30 min, and then the supernatant was incubated with HisPur Ni-NTA Resin (ThermoFisher) for 60 min at 4°C.

    Article Title: Structure and functional analysis of CDNF-BiP interaction reveal a role in endoplasmic reticulum proteostasis regulation
    Article Snippet: The lysate was centrifuged at 20,000 × g for 30 min, and then the supernatant was incubated with HisPur Ni-NTA Resin (Thermo Fisher) for 60 min at 4°C.

    Article Title: Supporting Information for Myxococcus xanthus Encapsulin Cargo Protein EncD is a Flavin-Binding Protein with Ferric Reductase Activity
    Article Snippet: The cleared supernatant for each preparation was incubated with 2 mL of HisPur Ni-NTA resin (Thermo Fisher Scientific) for 20 min at room temperature.

    Article Title: Structure and functional analysis of CDNF-BiP interaction reveal a role in endoplasmic reticulum proteostasis regulation
    Article Snippet: The sample was then incubated again with HisPur Ni-NTA Resin (ThermoFisher) for 60 min at 4°C, and the unbound fraction was collected.

    Lysis:

    Article Title:
    Article Snippet: Cell debris was removed by centrifugation (32,000 g, 1 h, 4 °C), and the supernatant was directly loaded onto a HisPur Ni‐ NTA resin (Thermo Scientific) column containing 15 mL of packed resin pre‐equilibrated with the lysis buffer.

    Article Title: Supplementary Information for Structural insights into the regulation of protein-arginine kinase McsB by McsA
    Article Snippet: After complete cleavage of 6×His-Trx-McsB into 6×His-Trx and McsB, the protein solution was dialyzed in buffer A for 3 h and passed through HisPur Ni-NTA resin (Thermo Fisher Scientific, Waltham, MA, USA) to remove the 6×His-Trx tag.

    Article Title:
    Article Snippet: His purification was conducted using the HisPur Ni-NTA resin from Thermo Fisher and the His-Spin buffers from Zymo.

    Article Title: Programmable translational inhibition by a molecular glue-oligonucleotide conjugate
    Article Snippet: After cleavage, the mixture was incubated with HisPur Ni-NTA resin (Thermo Fisher Scientific; 1 mL slurry per liter of culture) for 10 min, and the flow-through containing tag-free eIF4A1 was collected.

    Article Title: Structure and functional analysis of CDNF-BiP interaction reveal a role in endoplasmic reticulum proteostasis regulation
    Article Snippet: The lysate was centrifuged at 20,000 × g for 30 min, and then the supernatant was incubated with HisPur Ni-NTA Resin (ThermoFisher) for 60 min at 4°C.

    Article Title: Structure and functional analysis of CDNF-BiP interaction reveal a role in endoplasmic reticulum proteostasis regulation
    Article Snippet: The lysate was centrifuged at 20,000 × g for 30 min, and then the supernatant was incubated with HisPur Ni-NTA Resin (Thermo Fisher) for 60 min at 4°C.

    Article Title: Supporting Information for Myxococcus xanthus Encapsulin Cargo Protein EncD is a Flavin-Binding Protein with Ferric Reductase Activity
    Article Snippet: The cleared supernatant for each preparation was incubated with 2 mL of HisPur Ni-NTA resin (Thermo Fisher Scientific) for 20 min at room temperature.

    Article Title: Structure and functional analysis of CDNF-BiP interaction reveal a role in endoplasmic reticulum proteostasis regulation
    Article Snippet: The sample was then incubated again with HisPur Ni-NTA Resin (ThermoFisher) for 60 min at 4°C, and the unbound fraction was collected.



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